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Progesterone is a steroid hormone produced in the corpus luteum and the adrenal glands. Its main role is to prepare the body for and support a pregnancy. It is produced in increasing amounts in the second half of the menstrual cycle. Progesterone is normally tested on day 21 of your menstrual cycle to assess whether ovulation has taken place. Although progesterone is considered a female hormone, men also produce progesterone in the adrenal glands and the testes. Progesterone in men plays a role in testosterone production.